Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/105598
Title: A study comparing endogenous protoporphyrin IX induced by 5-ALA and ALA-methyl ester with exogenous PpIX and PpIX dimethyl ester in photodynamic diagnosis of human nasopharyngeal carcinoma xenografts
Authors: Manivasager, V.
Yee, K.K.L.
Heng, P.W.S. 
Soo, K.C.
Olivo, M.
Keywords: 5-aminolevulinic acid
5-aminolevulinic acid methyl ester
Photodynamic diagnosis
Protoporphyrin IX
Protoporphyrin IX dimethyl ester
Issue Date: Oct-2006
Source: Manivasager, V.,Yee, K.K.L.,Heng, P.W.S.,Soo, K.C.,Olivo, M. (2006-10). A study comparing endogenous protoporphyrin IX induced by 5-ALA and ALA-methyl ester with exogenous PpIX and PpIX dimethyl ester in photodynamic diagnosis of human nasopharyngeal carcinoma xenografts. International Journal of Oncology 29 (4) : 997-1002. ScholarBank@NUS Repository.
Abstract: 5-Aminolevulinic acid (5-ALA) and its esters have been under intense investigation to enhance the endogenous production of protoporphyrin IX (PpIX) in tumour cells for the purpose of photodynamic diagnosis. In this study we have investigated the use of exogenous PpIX and its dimethyl ester (PME) and compared the results with endogenous PpIX produced via 5-ALA and ALA methyl ester (AME) in poorly differentiated NPC/CNE-2 nasopharyngeal carcinoma cells in both in vivo and in vitro systems. All prodrugs and photosensitizers were administered to tumour bearing balb/c nude mice either intravenously or topically. In vitro results show that 5-ALA induced more PpIX fluorescence when compared with AME in NPC/CNE-2 cells and PME showed better uptake than PpIX. In vivo results show that exogenous PpIX and PME show promise as good candidates as photosensitizers for photodynamic diagnosis as they exhibit significant selectivity between tumour tissue and normal tissue at 3 h. Modification of delivery vehicle used for application of exogenous PpIX and PME could allow for rapid uptake; better selectivity and localization of the photosensitizer.
Source Title: International Journal of Oncology
URI: http://scholarbank.nus.edu.sg/handle/10635/105598
ISSN: 10196439
Appears in Collections:Staff Publications

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