Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/105588
Title: A rapid and sensitive liquid chromatography/tandem mass spectrometry method for determination of docetaxel in human plasma
Authors: Wang, L.Z. 
Goh, B.C.
Grigg, M.E.
Lee, S.C.
Khoo, Y.M.
Lee, H.S.
Issue Date: 2003
Source: Wang, L.Z.,Goh, B.C.,Grigg, M.E.,Lee, S.C.,Khoo, Y.M.,Lee, H.S. (2003). A rapid and sensitive liquid chromatography/tandem mass spectrometry method for determination of docetaxel in human plasma. Rapid Communications in Mass Spectrometry 17 (14) : 1548-1552. ScholarBank@NUS Repository.
Abstract: A novel, rapid and sensitive isocratic liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for quantification of docetaxel in human plasma with paclitaxel as internal standard. The high sensitivity and specificity of MS/MS detection enabled the use of a small volume of plasma (0.05 mL) and a simple liquid-liquid extraction procedure. Furthermore, a very short run-time (3 min) fulfilled the need for monitoring plasma levels of docetaxel from large-scale clinical studies. The calibration curve for docetaxel was linear over the range 5-1000 ng/mL with coefficients of correlation > 0.999 using only 0.05 mL plasma. The intra- and inter-day precisions (CV) of analysis were < 7%, and accuracy ranged from 96 to 110%. The applicability of the method was demonstrated in a pharmacokinetic study of a 1-h infusion of docetaxel with dosages of 75 mg/m2. Possible conjugated metabolites of docetaxel were not detected in patients' samples. © 2003 John Wiley & Sons, Ltd.
Source Title: Rapid Communications in Mass Spectrometry
URI: http://scholarbank.nus.edu.sg/handle/10635/105588
ISSN: 09514198
Appears in Collections:Staff Publications

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