Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.taap.2013.07.011
Title: A novel shogaol analog suppresses cancer cell invasion and inflammation, and displays cytoprotective effects through modulation of NF-κB and Nrf2-Keap1 signaling pathways
Authors: Gan, F.-F. 
Ling, H.
Ang, X.
Reddy, S.A.
Lee, S.S.-H.
Yang, H.
Tan, S.-H.
Hayes, J.D.
Chui, W.-K. 
Chew, E.-H. 
Keywords: Chemoprevention
Ginger
Michael acceptors
NF-κB
Nrf2-Keap1
Shogaols
Issue Date: 1-Nov-2013
Citation: Gan, F.-F., Ling, H., Ang, X., Reddy, S.A., Lee, S.S.-H., Yang, H., Tan, S.-H., Hayes, J.D., Chui, W.-K., Chew, E.-H. (2013-11-01). A novel shogaol analog suppresses cancer cell invasion and inflammation, and displays cytoprotective effects through modulation of NF-κB and Nrf2-Keap1 signaling pathways. Toxicology and Applied Pharmacology 272 (3) : 852-862. ScholarBank@NUS Repository. https://doi.org/10.1016/j.taap.2013.07.011
Abstract: Natural compounds containing vanilloid and Michael acceptor moieties appear to possess anti-cancer and chemopreventive properties. The ginger constituent shogaol represents one such compound. In this study, the anti-cancer potential of a synthetic novel shogaol analog 3-phenyl-3-shogaol (3-Ph-3-SG) was assessed by evaluating its effects on signaling pathways. At non-toxic concentrations, 3-Ph-3-SG suppressed cancer cell invasion in MDA-MB-231 and MCF-7 breast carcinoma cells through inhibition of PMA-activated MMP-9 expression. At similar concentrations, 3-Ph-3-SG reduced expression of the inflammatory mediators nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and prostanglandin-E2 (PGE2) in RAW 264.7 macrophage-like cells. Inhibition of cancer cell invasion and inflammation by 3-Ph-3-SG were mediated through suppression of the nuclear factor-kappaB (NF-κB) signaling pathway. The 3-Ph-3-SG also demonstrated cytoprotective effects by inducing the antioxidant response element (ARE)-driven genes NAD(P)H quinone oxidoreductase-1 (NQO1) and heme oxygenase-1 (HO-1). Cytoprotection by 3-Ph-3-SG was achieved at least partly through modification of cysteine residues in the E3 ubiquitin ligase substrate adaptor Kelch-like ECH-associated protein 1 (Keap1), which resulted in accumulation of transcription factor NF-E2 p45-related factor 2 (Nrf2). The activities of 3-Ph-3-SG were comparable to those of 6-shogaol, the most abundant naturally-occurring shogaol, and stronger than those of 4-hydroxyl-null deshydroxy-3-phenyl-3-shogaol, which attested the importance of the 4-hydroxy substituent in the vanilloid moiety for bioactivity. In summary, 3-Ph-3-SG is shown to possess activities that modulate stress-associated pathways relevant to multiple steps in carcinogenesis. Therefore, it warrants further investigation of this compound as a promising candidate for use in chemotherapeutic and chemopreventive strategies. © 2013 Elsevier Inc.
Source Title: Toxicology and Applied Pharmacology
URI: http://scholarbank.nus.edu.sg/handle/10635/105578
ISSN: 0041008X
DOI: 10.1016/j.taap.2013.07.011
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