Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/102206
Title: Development of a trivalent transgenic watermelon BH-1 resistant to virus infections
Authors: Niu, S.-N.
Huang, X.-S.
Wong, S.-M. 
Yu, J.-L.
Zhao, F.-X.
Wang, S.-Y.
Zhai, G.-M.
Shi, F.-S.
Cai, X.-Y.
Cao, Y.-G.
Xu, Y.
Zheng, F.
Wang, X.-H.
Keywords: Gene transformation
Resistance to virus infection
Virus diseases
Watermelon
Issue Date: 2007
Source: Niu, S.-N.,Huang, X.-S.,Wong, S.-M.,Yu, J.-L.,Zhao, F.-X.,Wang, S.-Y.,Zhai, G.-M.,Shi, F.-S.,Cai, X.-Y.,Cao, Y.-G.,Xu, Y.,Zheng, F.,Wang, X.-H. (2007). Development of a trivalent transgenic watermelon BH-1 resistant to virus infections. Acta Horticulturae 731 : 71-75. ScholarBank@NUS Repository.
Abstract: By using the coat protein gene of WMV-2, and replicase genes of ZYMV and CMV, a trivalent expression vector was constructed for transformation of watermelon plants with Agrobacterium tumefaciens. The transgenes were confirmed in progenies of T1, T2 and T3 generations by PCR-Southern blot analysis. By mechanical inoculation in greenhouse and field trial, the transgenic lines showed resistance to virus infections in relatively high level and the resistant transgenic line BH-1 was selected successfully.
Source Title: Acta Horticulturae
URI: http://scholarbank.nus.edu.sg/handle/10635/102206
ISSN: 05677572
Appears in Collections:Staff Publications

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