Please use this identifier to cite or link to this item:
Title: Ultrastructural study of Golgi duplication in Trypanosoma brucei
Authors: Yelinek, J.T.
He, C.Y. 
Warren, G.
Keywords: 3D reconstruction
Electron microscopy
ER exit site
Trypanosoma brucei
Issue Date: 2009
Source: Yelinek, J.T., He, C.Y., Warren, G. (2009). Ultrastructural study of Golgi duplication in Trypanosoma brucei. Traffic 10 (3) : 300-306. ScholarBank@NUS Repository.
Abstract: Golgi duplication in the protozoan parasite Trypanosoma brucei has been tracked using serial thin section three-dimensional reconstructions of transmission electron micrographs. The old Golgi maintains a constant size (∼0.060 μm3) throughout the cell cycle. A morphologically identifiable new Golgi appears at ∼0.20 of the cell cycle (defined by the size of the nucleus and lasting about 9 h) and grows from ∼0.018 μm3 until it is the same size as the old Golgi (by ∼0.55 of the cell cycle). Morphologically identifiable late Golgi appear at ∼0.58 of the cell cycle, but their volume (∼0.036 μm3) did not change significantly. Cryoimmunoelectron microscopy was used to identify candidates for the earliest new Golgi structures, and these comprised clusters of vesicles containing Golgi reassembly stacking protein (GRASP) near an endoplasmic reticulum exit site. These results, combined with earlier fluorescence data, suggest that the new Golgi begins functioning before cisternal stacks are formed. © 2009 The Authors Journal compilation © 2009 Blackwell Munksgaard.
Source Title: Traffic
ISSN: 13989219
DOI: 10.1111/j.1600-0854.2008.00873.x
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Mar 6, 2018


checked on Jan 23, 2018

Page view(s)

checked on Mar 11, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.