Please use this identifier to cite or link to this item:
|Title:||Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus|
|Authors:||Zhang, X. |
|Source:||Zhang, X.,Huang, C.,Xu, X.,Hew, C.L. (2002). Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus. Journal of General Virology 83 (2) : 471-477. ScholarBank@NUS Repository.|
|Abstract:||White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. In the present study, an open reading frame (termed the p22 gene) was revealed from a WSSV cDNA library. The gene was expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli and purified. Specific antibody was raised using the purified fusion protein (GST-P22). Temporal analysis showed that the p22 gene was a late gene. After binding between purified WSSV virions and anti-GST-P22 IgG followed by labelling with gold-labelled secondary antibody, the gold particles, under a transmission electron microscope, could be found along the outer envelope of WSSV virions. This experiment suggests that the p22 gene encodes an envelope protein of the virus.|
|Source Title:||Journal of General Virology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 14, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.