Please use this identifier to cite or link to this item:
|Title:||Temporary increase in plasma membrane tension coordinates the activation of exocytosis and contraction during cell spreading|
|Citation:||Gauthier, N.C., Fardin, M.A., Roca-Cusachs, P., Sheetz, M.P. (2011-08-30). Temporary increase in plasma membrane tension coordinates the activation of exocytosis and contraction during cell spreading. Proceedings of the National Academy of Sciences of the United States of America 108 (35) : 14467-14472. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.1105845108|
|Abstract:||Cell migration and spreading involve the coordination of membrane trafficking, actomyosin contraction, and modifications to plasma membrane tension and area. The biochemical or biophysical basis for this coordination is however unknown. In this study, we show that during cell spreading, lamellipodia protrusion flattens plasma membrane folds and blebs and, once the plasma membrane area is depleted, there is a temporary increase in membrane tension by over twofold that is followed by activation of exocytosis and myosin contraction. Further, an artificial increase in plasma membrane tension stopped lamellipodia protrusion and activated an exocytotic burst. Subsequent decrease in tension restored spreading with activation of contraction. Conversely, blebbistatin inhibition of actomyosin contraction resulted in an even greater increase in plasma membrane tension and exocytosis activation. This spatiotemporal synchronization indicates that membrane tension is the signal that coordinates membrane trafficking, actomyosin contraction, and plasma membrane area change. We suggest that cells use plasma membrane tension as a global physical parameter to control cell motility.|
|Source Title:||Proceedings of the National Academy of Sciences of the United States of America|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jun 17, 2018
WEB OF SCIENCETM
checked on May 16, 2018
checked on Jun 8, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.