Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0068759
Title: Super Resolution Microscopy Reveals that Caveolin-1 Is Required for Spatial Organization of CRFB1 and Subsequent Antiviral Signaling in Zebrafish
Authors: Gabor, K.A.
Stevens, C.R.
Pietraszewski, M.J.
Gould, T.J.
Shim, J.
Yoder, J.A.
Lam, S.H. 
Gong, Z. 
Hess, S.T.
Kim, C.H.
Issue Date: 9-Jul-2013
Citation: Gabor, K.A., Stevens, C.R., Pietraszewski, M.J., Gould, T.J., Shim, J., Yoder, J.A., Lam, S.H., Gong, Z., Hess, S.T., Kim, C.H. (2013-07-09). Super Resolution Microscopy Reveals that Caveolin-1 Is Required for Spatial Organization of CRFB1 and Subsequent Antiviral Signaling in Zebrafish. PLoS ONE 8 (7) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0068759
Abstract: Understanding spatial distribution and dynamics of receptors within unperturbed membranes is essential for elucidating their role in antiviral signaling, but conventional studies of detergent-resistant membrane fractions cannot provide this information. Caveolae are integral to numerous signaling pathways and these membrane domains have been previously implicated in viral entry but not antiviral defense. This study shows, for the first time, the importance of spatio-temporal regulation of signaling receptors and the importance of the regulation of clustering for downstream signaling. A novel mechanism for virus evasion of host cell defenses is demonstrated through disruption of clusters of signaling molecules organized within caveolin-rich domains. Viral infection leads to a downregulation in Caveolin-1b (Cav-1b), disrupting clusters of CRFB1, a zebrafish type I interferon receptor (-R) subunit. Super-resolution microscopy has enabled the first single-molecule imaging of CRFB1 association with cav-1b-containing membrane domains. Strikingly, downregulation of Cav-1b, the major protein component of caveolae, caused CRFB1 clusters to disperse. Dispersal of CRFB1 clusters led to a suppressed antiviral immune response both in vitro and in vivo, through abrogation of downstream signaling. This response strongly suggests that CRFB1 organization within cav-1b-containing membrane domains is critical for IFN-mediated antiviral defense and presents a previously undescribed antiviral evasion strategy to alter IFN signaling and the antiviral immune response. © 2013 Gabor et al.
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/101789
ISSN: 19326203
DOI: 10.1371/journal.pone.0068759
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2013-super_resolution_microscopy_reveals_that-pub.pdf1.36 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

18
checked on Dec 12, 2018

WEB OF SCIENCETM
Citations

17
checked on Dec 12, 2018

Page view(s)

59
checked on Dec 7, 2018

Download(s)

4
checked on Dec 7, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.