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https://doi.org/10.1038/cr.2008.288
DC Field | Value | |
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dc.title | Structural basis for dsRNA recognition by NS1 protein of influenza A virus | |
dc.contributor.author | Cheng, A. | |
dc.contributor.author | Wong, S.M. | |
dc.contributor.author | Yuan, Y.A. | |
dc.date.accessioned | 2014-10-27T08:40:30Z | |
dc.date.available | 2014-10-27T08:40:30Z | |
dc.date.issued | 2009-02 | |
dc.identifier.citation | Cheng, A., Wong, S.M., Yuan, Y.A. (2009-02). Structural basis for dsRNA recognition by NS1 protein of influenza A virus. Cell Research 19 (2) : 187-195. ScholarBank@NUS Repository. https://doi.org/10.1038/cr.2008.288 | |
dc.identifier.issn | 10010602 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/101743 | |
dc.description.abstract | Influenza A viruses are important human pathogens causing periodic pandemic threats. Nonstructural protein 1 (NS1) protein of influenza A virus (NS1A) shields the virus against host defense. Here, we report the crystal structure of NS1A RNA-binding domain (RBD) bound to a double-stranded RNA (dsRNA) at 1.7. NS1A RBD forms a homodimer to recognize the major groove of A-form dsRNA in a length-independent mode by its conserved concave surface formed by dimeric anti-parallel α-helices. dsRNA is anchored by a pair of invariable arginines (Arg38) from both monomers by extensive hydrogen bonds. In accordance with the structural observation, isothermal titration calorimetry assay shows that the unique Arg38-Arg38 pair and two Arg35-Arg46 pairs are crucial for dsRNA binding, and that Ser42 and Thr49 are also important for dsRNA binding. Agrobacterium co-infiltration assay further supports that the unique Arg38 pair plays important roles in dsRNA binding in vivo. © 2009 IBCB. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/cr.2008.288 | |
dc.source | Scopus | |
dc.subject | Crystal structure | |
dc.subject | Influenza A virus | |
dc.subject | Nonstructural protein 1 | |
dc.subject | Protein-RNA complex | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1038/cr.2008.288 | |
dc.description.sourcetitle | Cell Research | |
dc.description.volume | 19 | |
dc.description.issue | 2 | |
dc.description.page | 187-195 | |
dc.identifier.isiut | 000263287500005 | |
Appears in Collections: | Staff Publications |
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