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Title: Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus)
Authors: Chen, S.-L.
Sha, Z.-X.
Ye, H.-Q.
Liu, Y.
Tian, Y.-S.
Hong, Y. 
Tang, Q.-S.
Keywords: Cell transplantation
Embryonic stem cells
Sea perch
Issue Date: Jan-2007
Citation: Chen, S.-L., Sha, Z.-X., Ye, H.-Q., Liu, Y., Tian, Y.-S., Hong, Y., Tang, Q.-S. (2007-01). Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus). Marine Biotechnology 9 (1) : 82-91. ScholarBank@NUS Repository.
Abstract: A stable GFP-expressing (GFP +LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP +LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP +LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP +LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP +LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP +LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry. © 2007 Springer Science+Business Media, Inc.
Source Title: Marine Biotechnology
ISSN: 14362228
DOI: 10.1007/s10126-006-6050-1
Appears in Collections:Staff Publications

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