Please use this identifier to cite or link to this item:
|Title:||Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus)|
Embryonic stem cells
|Citation:||Chen, S.-L., Sha, Z.-X., Ye, H.-Q., Liu, Y., Tian, Y.-S., Hong, Y., Tang, Q.-S. (2007-01). Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus). Marine Biotechnology 9 (1) : 82-91. ScholarBank@NUS Repository. https://doi.org/10.1007/s10126-006-6050-1|
|Abstract:||A stable GFP-expressing (GFP +LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP +LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP +LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP +LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP +LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP +LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry. © 2007 Springer Science+Business Media, Inc.|
|Source Title:||Marine Biotechnology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 16, 2018
WEB OF SCIENCETM
checked on Jun 20, 2018
checked on May 4, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.