Please use this identifier to cite or link to this item: https://doi.org/10.1242/dev.052456
DC FieldValue
dc.titlePlasma membrane-bound AGC3 kinases phosphorylate PIN auxin carriers at TPRXS(N/S) motifs to direct apical PIN recycling
dc.contributor.authorDhonukshe, P.
dc.contributor.authorHuang, F.
dc.contributor.authorGalvan-Ampudia, C.S.
dc.contributor.authorMähönen, A.P.
dc.contributor.authorKleine-Vehn, J.
dc.contributor.authorXu, J.
dc.contributor.authorQuint, A.
dc.contributor.authorPrasad, K.
dc.contributor.authorFriml, J.
dc.contributor.authorScheres, B.
dc.contributor.authorOffringa, R.
dc.date.accessioned2014-10-27T08:36:55Z
dc.date.available2014-10-27T08:36:55Z
dc.date.issued2010-10-01
dc.identifier.citationDhonukshe, P., Huang, F., Galvan-Ampudia, C.S., Mähönen, A.P., Kleine-Vehn, J., Xu, J., Quint, A., Prasad, K., Friml, J., Scheres, B., Offringa, R. (2010-10-01). Plasma membrane-bound AGC3 kinases phosphorylate PIN auxin carriers at TPRXS(N/S) motifs to direct apical PIN recycling. Development 137 (19) : 3245-3255. ScholarBank@NUS Repository. https://doi.org/10.1242/dev.052456
dc.identifier.issn09501991
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101412
dc.description.abstractPolar membrane cargo delivery is crucial for establishing cell polarity and for directional transport processes. In plants, polar trafficking mediates the dynamic asymmetric distribution of PIN FORMED (PIN) carriers, which drive polar cell-to-cell transport of the hormone auxin, thereby generating auxin maxima and minima that control development. The Arabidopsis PINOID (PID) protein kinase instructs apical PIN localization by phosphorylating PINs. Here, we identified the PID homologs WAG1 and WAG2 as new PIN polarity regulators. We show that the AGC3 kinases PID, WAG1 and WAG2, and not other plant AGC kinases, instruct recruitment of PINs into the apical recycling pathway by phosphorylating the middle serine in three conserved TPRXS(N/S) motifs within the PIN central hydrophilic loop. Our results put forward a model by which apolarly localized PID, WAG1 and WAG2 phosphorylate PINs at the plasma membrane after default non-polar PIN secretion, and trigger endocytosis-dependent apical PIN recycling. This phosphorylation-triggered apical PIN recycling competes with ARF-GEF GNOM-dependent basal recycling to promote apical PIN localization. In planta, expression domains of PID, WAG1 and WAG2 correlate with apical localization of PINs in those cell types, indicating the importance of these kinases for apical PIN localization. Our data show that by directing polar PIN localization and PIN-mediated polar auxin transport, the three AGC3 kinases redundantly regulate cotyledon development, root meristem size and gravitropic response, indicating their involvement in both programmed and adaptive plant development.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1242/dev.052456
dc.sourceScopus
dc.subjectArabidopsis
dc.subjectBasal and apical recycling
dc.subjectEndocytosis-related Rab5 pathway
dc.subjectPID
dc.subjectPIN efflux carrier
dc.subjectPolar auxin transport
dc.subjectPolar trafficking
dc.subjectTranscytosis
dc.subjectWAG1
dc.subjectWAG2
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1242/dev.052456
dc.description.sourcetitleDevelopment
dc.description.volume137
dc.description.issue19
dc.description.page3245-3255
dc.description.codenDEVPE
dc.identifier.isiut000281608200011
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.