Please use this identifier to cite or link to this item: https://doi.org/10.1093/pcp/pct026
Title: Overexpression of DOSOC1, an ortholog of arabidopsis SOC1, promotes flowering in the orchid dendrobium chao parya smile
Authors: Ding, L.
Wang, Y.
Yu, H. 
Keywords: Arabidopsis
Flowering time
MADS-box gene
Orchid
SOC1
Issue Date: Apr-2013
Citation: Ding, L., Wang, Y., Yu, H. (2013-04). Overexpression of DOSOC1, an ortholog of arabidopsis SOC1, promotes flowering in the orchid dendrobium chao parya smile. Plant and Cell Physiology 54 (4) : 595-608. ScholarBank@NUS Repository. https://doi.org/10.1093/pcp/pct026
Abstract: SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) encodes a MADS-box protein that plays an essential role in integrating multiple flowering signals to regulate the transition from vegetative to reproductive development in the model plant Arabidopsis. Although SOC1-like genes have been isolated in various angiosperms, its orthologs in Orchidaceae, one of the largest families of flowering plants, are so far unknown. To investigate the regulatory mechanisms of flowering time control in orchids, we isolated a SOC1-like gene, DOSOC1, from Dendrobium Chao Praya Smile. DOSOC1 was highly expressed in reproductive organs, including inflorescence apices, pedicels, floral buds and open flowers. Its expression significantly increased in whole plantlets during the transition from vegetative to reproductive development, which usually occurred after 8 weeks of culture in Dendrobium Chao Praya Smile. In the shoot apex at the floral transitional stage, DOSOC1 was particularly expressed in emerging floral meristems. Overexpression of DOSOC1 in wild-type Arabidopsis plants resulted in early flowering, which was coupled with the up-regulation of two other flowering promoters, AGAMOUS-LIKE 24 and LEAFY. In addition, overexpression of DOSOC1 was able partially to complement the late-flowering phenotype of Arabidopsis soc1-2 loss-of-function mutants. Furthermore, we successfully created seven 35S:DOSOC1 transgenic Dendrobium orchid lines, which consistently exhibited earlier flowering than wild-type orchids. Our results suggest that SOC1-like genes play an evolutionarily conserved role in promoting flowering in the Orchidaceae family, and that DOSOC1 isolated from Dendrobium Chao Praya Smile could serve as an important target for genetic manipulation of flowering time in orchids. © 2013 The Author 2013. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Source Title: Plant and Cell Physiology
URI: http://scholarbank.nus.edu.sg/handle/10635/101340
ISSN: 00320781
DOI: 10.1093/pcp/pct026
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

27
checked on Jun 16, 2018

WEB OF SCIENCETM
Citations

27
checked on May 29, 2018

Page view(s)

31
checked on Feb 25, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.