Please use this identifier to cite or link to this item:
Title: Molecular rotors as conditionally fluorescent labels for rapid detection of biomolecular interactions
Authors: Goh, W.L.
Lee, M.Y.
Joseph, T.L.
Quah, S.T.
Brown, C.J.
Verma, C. 
Brenner, S.
Ghadessy, F.J.
Teo, Y.N.
Issue Date: 30-Apr-2014
Citation: Goh, W.L., Lee, M.Y., Joseph, T.L., Quah, S.T., Brown, C.J., Verma, C., Brenner, S., Ghadessy, F.J., Teo, Y.N. (2014-04-30). Molecular rotors as conditionally fluorescent labels for rapid detection of biomolecular interactions. Journal of the American Chemical Society 136 (17) : 6159-6162. ScholarBank@NUS Repository.
Abstract: We demonstrate the use of fluorescent molecular rotors as probes for detecting biomolecular interactions, specifically peptide-protein interactions. Molecular rotors undergo twisted intramolecular charge transfer upon irradiation, relax via the nonradiative torsional relaxation pathway, and have been typically used as viscosity probes. Their utility as a tool for detecting specific biomolecular interactions has not been explored. Using the well characterized p53-Mdm2 interaction as a model system, we designed a 9-(2-carboxy-2-cyanovinyl) julolidine-based p53 peptide reporter, JP1-R, which fluoresces conditionally only upon Mdm2 binding. The reporter was used in a rapid, homogeneous assay to screen a fragment library for antagonists of the p53-Mdm2 interaction, and several inhibitors were identified. Subsequent validation of these hits using established secondary assays suggests increased sensitivity afforded by JP1-R. The fluorescence of molecular rotors contingent upon target binding makes them a versatile tool for detecting specific biomolecular interactions. © 2014 American Chemical Society.
Source Title: Journal of the American Chemical Society
ISSN: 15205126
DOI: 10.1021/ja413031h
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Nov 14, 2018


checked on Nov 6, 2018

Page view(s)

checked on Nov 9, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.