Please use this identifier to cite or link to this item: https://doi.org/10.1101/gad.1985511
Title: Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b
Authors: Wang, M.
Soyano, T.
MacHida, S.
Yang, J.-Y.
Jung, C.
Chua, N.-H.
Yuan, Y.A. 
Keywords: ADP ribosylation toxin
Agrobacterium protein 6b
Crystal structure
MiRNA machinery
MiRNA phenotype
Issue Date: 1-Jan-2011
Citation: Wang, M., Soyano, T., MacHida, S., Yang, J.-Y., Jung, C., Chua, N.-H., Yuan, Y.A. (2011-01-01). Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b. Genes and Development 25 (1) : 64-76. ScholarBank@NUS Repository. https://doi.org/10.1101/gad.1985511
Abstract: The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 Å, Agrobacterium vitis AB6b at 1.65 Å, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 Å. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity. Copyright © 2011 by Cold Spring Harbor Laboratory Press.
Source Title: Genes and Development
URI: http://scholarbank.nus.edu.sg/handle/10635/101144
ISSN: 08909369
DOI: 10.1101/gad.1985511
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