Please use this identifier to cite or link to this item:
|Title:||Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b|
|Keywords:||ADP ribosylation toxin|
Agrobacterium protein 6b
|Citation:||Wang, M., Soyano, T., MacHida, S., Yang, J.-Y., Jung, C., Chua, N.-H., Yuan, Y.A. (2011-01-01). Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b. Genes and Development 25 (1) : 64-76. ScholarBank@NUS Repository. https://doi.org/10.1101/gad.1985511|
|Abstract:||The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 Å, Agrobacterium vitis AB6b at 1.65 Å, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 Å. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity. Copyright © 2011 by Cold Spring Harbor Laboratory Press.|
|Source Title:||Genes and Development|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 20, 2018
WEB OF SCIENCETM
checked on Sep 5, 2018
checked on Aug 17, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.