Please use this identifier to cite or link to this item: https://doi.org/10.1007/s11262-004-5629-7
Title: Molecular cloning, DNA sequence analysis, and expression of cDNA sequence of RNA genomic segment 6 (S6) that encodes a viral outer capsid protein of threadfin aquareovirus (TFV)
Authors: Seng, E.K.
Fang, Q.
Sin, Y.M.
Lam, T.J. 
Keywords: Cloning
Outer capsid protein
Recombinant protein expression
Sequence analysis
Threadfin aquareovirus
Issue Date: Mar-2005
Citation: Seng, E.K., Fang, Q., Sin, Y.M., Lam, T.J. (2005-03). Molecular cloning, DNA sequence analysis, and expression of cDNA sequence of RNA genomic segment 6 (S6) that encodes a viral outer capsid protein of threadfin aquareovirus (TFV). Virus Genes 30 (2) : 209-221. ScholarBank@NUS Repository. https://doi.org/10.1007/s11262-004-5629-7
Abstract: The genome segment 6 (S6) of threadfin reovirus (TFV) was cloned and sequenced. The entire S6 nucleotide sequence is 2056 bp long with an open reading frame that encodes a protein of 653 amino acids. Sequence analysis of the TFV S6 genome revealed that the 5′-terminal sequence, GTTTTA and the 3′-terminal sequence, ATTCATC of the plus strand is common to other genome segments of TFV. The pentanucleotide, TCATC, at the 3′-terminal of the plus strand was also conserved in other reported isolates of Aquareovirus such as chum salmon reovirus (CSV), striped bass reovirus (SBR), grass carp reovirus (GCRV) and golden shiner reovirus (GSV) as well as to the 10 genome segments of mammalian reovirus (MRV). Blast results indicated that the TFV S6 gene segment sequence had high identity towards the CSV S6 gene sequence, which codes for the CSV outer coat protein. This implied that the TFV S6 gene segment codes for an outer capsid protein (OCP) of the virus. Amino acid sequence analysis of this TFV OCP sequence revealed the presence of a putative conserved asparagine-proline (Asn-Pro) protease cleavage site, which was found in all reported isolates of Aquareovirus as well as in the MRV μ1 protein. N-terminal sequencing of the corresponding S6 native protein obtained from purified TFV particles verified the presence of this cleavage site. Phylogenetic analysis of the TFV S6 protein revealed that TFV was closely related to CSV, from Aquareovirus species, ARV-A. Cloning of the TFV S6 gene sequence into an Escherichia coli expression host produced a recombinant protein that corresponded to the predicated size of the OCP of TFV. Immunization of mice using this recombinant outer capsid protein (rOCP) revealed that the protein was able to elicit an antibody response, thus indicating that the rOCP of TFV was immunogenic. © 2005 Springer Science+Business Media, Inc.
Source Title: Virus Genes
URI: http://scholarbank.nus.edu.sg/handle/10635/101131
ISSN: 09208569
DOI: 10.1007/s11262-004-5629-7
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

2
checked on Jul 14, 2018

WEB OF SCIENCETM
Citations

2
checked on Jun 19, 2018

Page view(s)

29
checked on Jun 8, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.