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|Title:||Modulation of SAMDC expression in Arabidopsis thaliana alters in vitro shoot organogenesis|
|Citation:||Hu, W.-W., Gong, H., Pua, E.-C. (2006-12). Modulation of SAMDC expression in Arabidopsis thaliana alters in vitro shoot organogenesis. Physiologia Plantarum 128 (4) : 740-750. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1399-3054.2006.00799.x|
|Abstract:||S-Adenosylmethionine decarboxylase (SAMDC, EC 220.127.116.11) is a rate-limiting enzyme in the biosynthesis of polyamines (PAs) from putrescine. To gain more insight into the role of PAs in shoot organogenesis, a reverse genetic approach has been used to study in vitro shoot organogenesis by manipulating SAMDC expression in Arabidopsis. Up- and downregulation of SAMDC expression was achieved by transferring sense, antisense and double-stranded Arabidopsis SAMDC complementary DNA constructs back into Arabidopsis via Agrobacterium tumefaciens. Results show that the biosynthesis of PAs and ethylene is mutually antagonistic by manipulation of SAMDC expression. Further results demonstrate that increased shoot organogenesis seems to be directly related to PA accumulation. This effect of PA may be further enhanced with reduced ethylene. It also suggests that spermidine is involved in the process of acquiring organogenesis competence through downregulation of ethylene production and shoot organogenesis, which might result from the concerted action of PAs and plant hormones such as auxin, cytokinins and ethylene. © Physiologia Plantarum 2006.|
|Source Title:||Physiologia Plantarum|
|Appears in Collections:||Staff Publications|
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