Please use this identifier to cite or link to this item:
|Title:||Millisecond timescale dynamics of human liver fatty acid binding protein: Testing of its relevance to the ligand entry process|
|Citation:||Long, D., Yang, D. (2010-06-16). Millisecond timescale dynamics of human liver fatty acid binding protein: Testing of its relevance to the ligand entry process. Biophysical Journal 98 (12) : 3054-3061. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bpj.2010.03.047|
|Abstract:||For over a decade, scientists have been attempting to know more about the conformational dynamics of fatty acid binding proteins (FABPs), to answer the puzzling question of how ligands could access the Internalized binding site(s). Conformational exchange of FABPs on the microsecond to millisecond timescales has been found in many FABPs and offers an important hypothesis for the ligand entry mechanism. Despite the potential significance, the validity of this hypothesis has not been verified yet. In this study, the slow dynamics of human liver fatty acid binding protein (hLFABP) that was shown previously to be highly flexible on millisecond timescales was quantitatively characterized in detail. In addition, the interaction between hLFABP and 1,8-ANS was studied using NMR spectroscopy, and the kinetic rate of ANS association to hLFABP was measured. We believe the current result excludes the possibility that the intrinsic millisecond dynamics of hLFABP represents a critical conformational reorganization process required for ligand entry, but implies that it may represent the exchange between the apo-state and a state resembling the singly-bound conformation. Furthermore, we suggest these results show that the ligandentry related functional dynamics could occur on the microsecond/ submicrosecond timescales, highly encouraging future computational studies on this topic. © 2010 by the Biophysical Society.|
|Source Title:||Biophysical Journal|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 16, 2018
WEB OF SCIENCETM
checked on Jun 26, 2018
checked on Feb 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.