Please use this identifier to cite or link to this item:
Title: Medaka vasa is required for migration but not survival of primordial germ cells
Authors: Li, M. 
Hong, N.
Xu, H. 
Yi, M. 
Li, C.
Gui, J.
Hong, Y. 
Keywords: Cell migration
Germ cell culture
Oryzias latipes
Primordial germ cell
Issue Date: May-2009
Source: Li, M., Hong, N., Xu, H., Yi, M., Li, C., Gui, J., Hong, Y. (2009-05). Medaka vasa is required for migration but not survival of primordial germ cells. Mechanisms of Development 126 (5-6) : 366-381. ScholarBank@NUS Repository.
Abstract: vasa is essential for germline development. However, the precise processes in which vasa involves vary considerably in diverse animal phyla. Here we show that vasa is required for primordial germ cell (PGC) migration in the medakafish. vasa knockdown by two morpholinos led to the PGC migration defect that was rescued by coinjection of vasa RNA. Interestingly, vasa knockdown did not alter the PGC number, identity, proliferation and motility even at ectopic locations. We established a cell culture system for tracing PGCs at the single cell level in vitro. In this culture system, control and morpholino-injected gastrulae produced the same PGC number and the same time course of PGC survival. Importantly, vasa-depleted PGCs in culture had similar motility and locomotion to normal PGCs. Expression patterns of wt1a, sdf1b and cxcr4b in migratory tissues remained unchanged by vasa knockdown. By chimera formation we show that PGCs from vasa-depleted blastulae failed to migrate properly in the normal environment, whereas control PGCs migrated normally in vasa-disrupted embryos. Furthermore, ectopic PGCs in vasa-depleted embryos also retained all the PGC properties examined. Taken together, medaka vasa is cell-autonomously required for PGC migration, but dispensable to PGC proliferation, motility, identity and survival. © 2009 Elsevier Ireland Ltd. All rights reserved.
Source Title: Mechanisms of Development
ISSN: 09254773
DOI: 10.1016/j.mod.2009.02.004
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Mar 6, 2018


checked on Mar 6, 2018

Page view(s)

checked on Feb 25, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.