Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0002850
Title: K-ras/PI3K-Akt signaling is essential for zebrafish hematopoiesis and angiogenesis
Authors: Liu, L. 
Zhu, S. 
Gong, Z. 
Low, B.C. 
Issue Date: 6-Aug-2008
Source: Liu, L., Zhu, S., Gong, Z., Low, B.C. (2008-08-06). K-ras/PI3K-Akt signaling is essential for zebrafish hematopoiesis and angiogenesis. PLoS ONE 3 (8) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0002850
Abstract: The RAS small GTPases orchestrate multiple cellular processes. Studies on knock-out mice showed the essential and sufficient role of K-RAS, but not N-RAS and H-RAS in embryonic development. However, many physiological functions of K-RAS in vivo remain unclear. Using wild-type and fli1:GFP transgenic zebrafish, we showed that K-ras-knockdown resulted in specific hematopoietic and angiogenic defects, including the impaired expression of erythroid-specific gene gatal and βe3-hemoglobin, reduced blood circulation and disorganized blood vessels. Expression of either K-rasC40 that links to phosphoinositide 3-kinase (P13K) activation, or Akt2 that acts downstream of P13K, could rescue both hematopoietic and angiogenic defects in the K-ras knockdown. Consistently, the functional rescue by k-ras mRNA was significantly suppressed by wortmannin, a P13K-specific inhibitor. Our results provide direct evidence that P13K-Akt plays a crucial role in mediating K-ras signaling during hematopoiesis and angiogenesis in vivo, thus offering new targets and alternative vertebrate model for studying these processes and their related diseases. © 2008 Liu et al.
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/101000
ISSN: 19326203
DOI: 10.1371/journal.pone.0002850
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