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|Title:||Intracellular delivery of biologically active proteins with peptide-based carriers|
|Source:||Lo, S.L.,Wang, S. (2013). Intracellular delivery of biologically active proteins with peptide-based carriers. Methods in Molecular Biology 991 : 275-280. ScholarBank@NUS Repository. https://doi.org/10.1007/978-1-62703-336-7_25|
|Abstract:||The medical applications of protein-based therapeutics are hampered by low bioavailability associated with inefficient intracellular delivery. Various delivery materials have been developed and tested to interact with protein cargos in a manner of stabilizing proteins extracellularly and facilitating cellular uptake of proteins, thus enhancing delivery efficiency. Peptides that can form stable complexes with proteins through non-covalent interaction appear to be a promising tool to improve intracellular delivery of proteins. Here we describe the preparation of complexes formed between β-galactosidase and peptide-based carrier, protein transfer of the complexes, and the methods to evaluate delivery efficiency qualitatively and quantitatively. © 2013 Springer Science+Business Media New York.|
|Source Title:||Methods in Molecular Biology|
|Appears in Collections:||Staff Publications|
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