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|Title:||Identification of proteins differentially expressed between capillary endothelial cells of hepatocellular carcinoma and normal liver in an orthotopic rat tumor model using 2-D DIGE|
|Authors:||Jia, J. |
Sinusoid endothelial cell
Tumor endothelial cell
|Citation:||Jia, J., Wang, J., Teh, M., Sun, W., Zhang, J., Kee, I., Chow, P.K.-H., Liang, R.C.M.-Y., Chung, M.C.M., Ge, R. (2010-01). Identification of proteins differentially expressed between capillary endothelial cells of hepatocellular carcinoma and normal liver in an orthotopic rat tumor model using 2-D DIGE. Proteomics 10 (2) : 224-234. ScholarBank@NUS Repository. https://doi.org/10.1002/pmic.200900607|
|Abstract:||Hepatocellular carcinoma (HCC) is one of the deadliest cancers with few treatment options. It is a hypervascular tumor in which angiogenesis plays a critical role in its progression. Tumor capillary endothelial cells (TECs) in HCC are known to originate from liver sinusoid endothelial cells, which then go through a capillarization process to become morphologically as well as functionally different TECs. In this work, we investigated proteins differentially expressed between freshly isolated TECs and sinusoid endothelial cells from well-formed rat HCC using 2-D DIGE coupled with MALDI-TOF/TOF MS. Thirty-eight unique proteins were identified to be differentially expressed more than twofold between the two endothelial cell types. Amongst the differentially expressed proteins, two novel endothelial markers, EH domain-containing protein 3 and galectin-3, were confirmed by Western blot and immunohistochemistry in both rat and human HCC samples. We showed that EH domain-containing protein 3 is significantly down-regulated in TECs, but galectin-3 is up-regulated. We propose possible roles of these two proteins in tumor vessel development in HCC. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.|
|Appears in Collections:||Staff Publications|
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