Please use this identifier to cite or link to this item: https://doi.org/10.1042/BJ20081588
Title: Identification and characterization of the lipid-binding property of GrlR, a locus of enterocyte effacement regulator
Authors: Jobichen, C. 
Fernandis, A.Z.
Velazquez-Campoy, A.
Leung, K.Y. 
Mok, Y.-K. 
Wenk, M.R.
Sivaraman, J. 
Keywords: β-barrel protein
Global regulator of LEE (locus of enterocyte effacement) repressor (Gr1R)
Lipid
Lipocalin
Type III secretion system (T3SS)
Issue Date: 1-Jun-2009
Citation: Jobichen, C., Fernandis, A.Z., Velazquez-Campoy, A., Leung, K.Y., Mok, Y.-K., Wenk, M.R., Sivaraman, J. (2009-06-01). Identification and characterization of the lipid-binding property of GrlR, a locus of enterocyte effacement regulator. Biochemical Journal 420 (2) : 191-199. ScholarBank@NUS Repository. https://doi.org/10.1042/BJ20081588
Abstract: Lipocalins are a broad family of proteins identified initially in eukaryotes and more recently in Gram-negative bacteria. The functions of lipocalin or lipid-binding proteins are often elusive and very diverse. Recently, we have determined the structure of GrlR (global regulator of LEE repressor), which plays a key role in the regulation of LEE (locus of enterocyte effacement) proteins. GrlR adopts a lipocalin-like fold that is composed of an eight-stranded β-barrel followed by an α-helix at the C-terminus. GrlR has a highly hydrophobic cavity region and could be a potential transporter of lipophilic molecules. To verify this hypothesis, we carried out structure-based analysis of GrlR, determined the structure of the lipid-GrlR complex and measured the binding of lipid to recombinant GrlR by ITC (isothermal titration calorimetry). In addition, we identified phosphatidylglycerol and phosphatidylethanolamine as the endogenously bound lipid species of GrlR using electrospray-ionization MS. Furthermore, we have shown that the lipid-binding property of GrlR is similar to that of its closest lipocalin structural homologue, β-lactoglobulin. Our studies demonstrate the hitherto unknown lipid-binding property of GrlR. © The Authors Journal compilation.
Source Title: Biochemical Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/100862
ISSN: 02646021
DOI: 10.1042/BJ20081588
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

14
checked on Jun 14, 2018

WEB OF SCIENCETM
Citations

14
checked on May 7, 2018

Page view(s)

52
checked on Jun 1, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.