Please use this identifier to cite or link to this item:
https://doi.org/10.1099/vir.0.80345-0
DC Field | Value | |
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dc.title | Host-specific encapsidation of a defective RNA 3 of Cucumber mosaic virus | |
dc.contributor.author | Kaplan, I.B. | |
dc.contributor.author | Lee, K.-C. | |
dc.contributor.author | Canto, T. | |
dc.contributor.author | Wong, S.-M. | |
dc.contributor.author | Palukaitis, P. | |
dc.date.accessioned | 2014-10-27T08:30:42Z | |
dc.date.available | 2014-10-27T08:30:42Z | |
dc.date.issued | 2004-12 | |
dc.identifier.citation | Kaplan, I.B., Lee, K.-C., Canto, T., Wong, S.-M., Palukaitis, P. (2004-12). Host-specific encapsidation of a defective RNA 3 of Cucumber mosaic virus. Journal of General Virology 85 (12) : 3757-3763. ScholarBank@NUS Repository. https://doi.org/10.1099/vir.0.80345-0 | |
dc.identifier.issn | 00221317 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/100844 | |
dc.description.abstract | Defective (D) RNAs were generated in tobacco upon passage of two isolates of Cucumber mosaic virus (CMV) initially derived from RNA transcripts of cDNA clones. In both cases, the D RNA was derived by a single in-frame deletion of either 339 or 411 nt within the 3a gene of Fny-CMV RNA 3 or M-CMV RNA 3, respectively. The generation of D RNAs was rare and occurred with two CMV isolates, the virions of which were known to differ in physico-chemical properties. The Fny-CMV D RNA 3, designated D RNA 3-1, was maintained by passage together with Fny-CMV in tobacco, but was lost by passage in squash. D RNA 3-1 accumulated in the inoculated squash cotyledons but not in upper, systemically infected leaves. Virions purified from infected squash cotyledons or leaf mesophyll protoplasts did not contain D RNA 3-1. Therefore, the failure of D RNA 3-1 to accumulate in squash leaves systemically infected by CMV was due to a lack of encapsidation of the D RNA 3-1 and movement out of the inoculated leaves. © 2004 SGM. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1099/vir.0.80345-0 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1099/vir.0.80345-0 | |
dc.description.sourcetitle | Journal of General Virology | |
dc.description.volume | 85 | |
dc.description.issue | 12 | |
dc.description.page | 3757-3763 | |
dc.description.coden | JGVIA | |
dc.identifier.isiut | 000225442500028 | |
Appears in Collections: | Staff Publications |
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