Please use this identifier to cite or link to this item: https://doi.org/10.1021/ja045300l
Title: General method for suppression of diagonal peaks in heteronuclear-edited NOESY spectroscopy
Authors: Wu, J.
Fan, J.-S. 
Pascal, S.M.
Yang, D. 
Issue Date: 24-Nov-2004
Citation: Wu, J., Fan, J.-S., Pascal, S.M., Yang, D. (2004-11-24). General method for suppression of diagonal peaks in heteronuclear-edited NOESY spectroscopy. Journal of the American Chemical Society 126 (46) : 15018-15019. ScholarBank@NUS Repository. https://doi.org/10.1021/ja045300l
Abstract: A novel approach is proposed for suppression of diagonal peaks in 15N- and 13C-edited NOESY spectroscopy based on subtracting a spectrum with only diagonal peaks from the conventional NOESY spectrum. This method can be applied to most heteronuclear-edited NOESY experiments. It is far more sensitive than the TROSY-based approach for biomolecules with little TROSY effect, and nearly complete suppression of diagonal peaks can be achieved. The method has been demonstrated on samples of 15N-labeled calmodulin (17 kDa), 13C-labeled DdCAD-1 (24 kDa), and 13C-labeled hemoglobin (65 kDa), showing that cross-peaks very close to diagonals can be assigned reliably based on the difference spectra. Copyright © 2004 American Chemical Society.
Source Title: Journal of the American Chemical Society
URI: http://scholarbank.nus.edu.sg/handle/10635/100740
ISSN: 00027863
DOI: 10.1021/ja045300l
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