Please use this identifier to cite or link to this item: https://doi.org/10.1002/jps.10149
Title: Formulation pH modulates the interaction of insulin with chitosan nanoparticles
Authors: Zengshuan, Ma.
Yeoh, H.H. 
Lim, L.-Y. 
Keywords: Association efficiency
Chitosan
Insulin
Nanoparticles
PH
Recovery
Issue Date: 2002
Citation: Zengshuan, Ma., Yeoh, H.H., Lim, L.-Y. (2002). Formulation pH modulates the interaction of insulin with chitosan nanoparticles. Journal of Pharmaceutical Sciences 91 (6) : 1396-1404. ScholarBank@NUS Repository. https://doi.org/10.1002/jps.10149
Abstract: Previous studies on chitosan-insulin nanoparticles have reported diverse encapsulation efficiency and insulin release profiles despite similar formulation and preparation procedures. This study examined the efficiency and mechanism of association of insulin with chitosan nanoparticles in the pH range of 2.3 to 6.3. Nanoparticles of 237 to 253 nm were prepared by ionotropic gelation of chitosan with tripolyphosphate counterions. Insulin was quantified by an RP-HPLC method. The insulin association efficiency (AE) spanned a broad range from 2 to 85%, and was highly sensitive to formulation pH. Highest AE was measured at insulin loading concentrations ≥ 4.28 U/mL and pH 6.1, close to the pI of native insulin and the pKa of chitosan. This association, attributed to physical adsorption of insulin through hydrophobic interactions with chitosan, was labile, and the associated insulin rapidly and completely released by dilution of the nanoparticles in aqueous media of pH 2 to 7.4. AE obtained at pH 5.3 was less than half that measured at pH 6.1 at corresponding insulin concentration, but the association at pH 5.3 appeared to be based on stronger interactions, because the release of insulin was pH-dependent and recovery was less than 25% even upon disintegration of the chitosan matrix. Interaction of insulin with the chitosan nanoparticles rendered the protein more susceptible to acid and enzymatic hydrolyses, the effects being more predominant in nanoparticles prepared at pH 5.3 than at pH 6.1. © 2002 Wiley-Liss, Inc.
Source Title: Journal of Pharmaceutical Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/100706
ISSN: 00223549
DOI: 10.1002/jps.10149
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

113
checked on Dec 13, 2018

WEB OF SCIENCETM
Citations

118
checked on Nov 27, 2018

Page view(s)

43
checked on Nov 16, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.