Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/100653
Title: Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii
Authors: Huynh, F.
Tan, T.-C.
Swaminathan, K. 
Patel, B.K.C.
Issue Date: 2005
Citation: Huynh, F., Tan, T.-C., Swaminathan, K., Patel, B.K.C. (2005). Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61 (1) : 116-117. ScholarBank@NUS Repository.
Abstract: This is the first report of the crystallization of a sucrose phosphate synthase (SPS; EC 2.4.1.14). It also constitutes the first study of a sucrose phosphate synthase from a non-photosynthetic thermohalophilic anaerobic bacterium, Halothermothrix orenii. The purified recombinant spsA protein has been crystallized in the monoclinic space group C2, with unit-cell parameters a = 154.2, b = 47.9, c = 72.3 Å, β = 103.16°, using the hanging-drop vapour-diffusion method. The crystal diffracts X-rays to a resolution limit of 3.01 Å. Heavy-metal and halide-soaking trials are currently in progress to solve the structure. © 2005 International Union of Crystallography All rights reserved.
Source Title: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/100653
ISSN: 17443091
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