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https://doi.org/10.1007/s002990000268
DC Field | Value | |
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dc.title | Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid-cycling Brassica napus | |
dc.contributor.author | Koh, W.L. | |
dc.contributor.author | Loh, C.S. | |
dc.date.accessioned | 2014-10-27T08:26:16Z | |
dc.date.available | 2014-10-27T08:26:16Z | |
dc.date.issued | 2000 | |
dc.identifier.citation | Koh, W.L., Loh, C.S. (2000). Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid-cycling Brassica napus. Plant Cell Reports 19 (12) : 1177-1183. ScholarBank@NUS Repository. https://doi.org/10.1007/s002990000268 | |
dc.identifier.issn | 07217714 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/100475 | |
dc.description.abstract | A simple method to induce somatic embryogenesis from seeds of rapid-cycling Brassica napus is described. Seedlings cultured on Murashige and Skoog (MS) basal medium produced somatic embryos directly on hypocotyls and cotyledons after 2 to 3 subcultures onto the same medium. A low pH of the medium (3.5-5) was more conducive to somatic embryogenesis than a higher pH (6 and 7). Embryogenic potential of the seeds was inversely correlated to seed age: about 41-68% of immature seeds between the ages of 14 and 28 days after pollination (DAP) formed somatic embryos compared to 0-11% of the seeds obtained 29-37 DAP. About 54% of the somatic embryos produced secondary embryos after subculturing onto the same medium. The embryogenic potential of the cultures has been maintained on MS basal medium for 2 years (12 generations) without diminution. Up to 75% of the secondary embryos developed into plantlets on MS medium enriched with 10-6 M zeatin, and 40% of these produced flowers when transferred to an optimised flower-induction medium. Viable seeds were produced in self-pollinated in vitro flowers. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s002990000268 | |
dc.source | Scopus | |
dc.subject | Brassica napus | |
dc.subject | In vitro howering | |
dc.subject | Rapid-cycling | |
dc.subject | Regeneration | |
dc.subject | Secondary embryogenesis | |
dc.subject | Somatic embryogenesis | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1007/s002990000268 | |
dc.description.sourcetitle | Plant Cell Reports | |
dc.description.volume | 19 | |
dc.description.issue | 12 | |
dc.description.page | 1177-1183 | |
dc.description.coden | PCRPD | |
dc.identifier.isiut | 000166219600008 | |
Appears in Collections: | Staff Publications |
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