Please use this identifier to cite or link to this item:
|Title:||Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid-cycling Brassica napus|
In vitro howering
|Citation:||Koh, W.L., Loh, C.S. (2000). Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid-cycling Brassica napus. Plant Cell Reports 19 (12) : 1177-1183. ScholarBank@NUS Repository. https://doi.org/10.1007/s002990000268|
|Abstract:||A simple method to induce somatic embryogenesis from seeds of rapid-cycling Brassica napus is described. Seedlings cultured on Murashige and Skoog (MS) basal medium produced somatic embryos directly on hypocotyls and cotyledons after 2 to 3 subcultures onto the same medium. A low pH of the medium (3.5-5) was more conducive to somatic embryogenesis than a higher pH (6 and 7). Embryogenic potential of the seeds was inversely correlated to seed age: about 41-68% of immature seeds between the ages of 14 and 28 days after pollination (DAP) formed somatic embryos compared to 0-11% of the seeds obtained 29-37 DAP. About 54% of the somatic embryos produced secondary embryos after subculturing onto the same medium. The embryogenic potential of the cultures has been maintained on MS basal medium for 2 years (12 generations) without diminution. Up to 75% of the secondary embryos developed into plantlets on MS medium enriched with 10-6 M zeatin, and 40% of these produced flowers when transferred to an optimised flower-induction medium. Viable seeds were produced in self-pollinated in vitro flowers.|
|Source Title:||Plant Cell Reports|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jun 17, 2018
WEB OF SCIENCETM
checked on May 16, 2018
checked on Jun 22, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.