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Title: Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid-cycling Brassica napus
Authors: Koh, W.L.
Loh, C.S. 
Keywords: Brassica napus
In vitro howering
Secondary embryogenesis
Somatic embryogenesis
Issue Date: 2000
Citation: Koh, W.L., Loh, C.S. (2000). Direct somatic embryogenesis, plant regeneration and in vitro flowering in rapid-cycling Brassica napus. Plant Cell Reports 19 (12) : 1177-1183. ScholarBank@NUS Repository.
Abstract: A simple method to induce somatic embryogenesis from seeds of rapid-cycling Brassica napus is described. Seedlings cultured on Murashige and Skoog (MS) basal medium produced somatic embryos directly on hypocotyls and cotyledons after 2 to 3 subcultures onto the same medium. A low pH of the medium (3.5-5) was more conducive to somatic embryogenesis than a higher pH (6 and 7). Embryogenic potential of the seeds was inversely correlated to seed age: about 41-68% of immature seeds between the ages of 14 and 28 days after pollination (DAP) formed somatic embryos compared to 0-11% of the seeds obtained 29-37 DAP. About 54% of the somatic embryos produced secondary embryos after subculturing onto the same medium. The embryogenic potential of the cultures has been maintained on MS basal medium for 2 years (12 generations) without diminution. Up to 75% of the secondary embryos developed into plantlets on MS medium enriched with 10-6 M zeatin, and 40% of these produced flowers when transferred to an optimised flower-induction medium. Viable seeds were produced in self-pollinated in vitro flowers.
Source Title: Plant Cell Reports
ISSN: 07217714
DOI: 10.1007/s002990000268
Appears in Collections:Staff Publications

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