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Title: | Direct shoot formation and plant regeneration from cotyledon explants of rapid-cycling Brassica rapa | Authors: | Teo, W. Lakshmanan, P. Kumar, P. Goh, C.-J. Swarup, S. |
Keywords: | Aminoethoxyvinylglycine Rooting Shoot regeneration |
Issue Date: | Oct-1997 | Citation: | Teo, W.,Lakshmanan, P.,Kumar, P.,Goh, C.-J.,Swarup, S. (1997-10). Direct shoot formation and plant regeneration from cotyledon explants of rapid-cycling Brassica rapa. In Vitro Cellular and Developmental Biology - Plant 33 (4) : 288-292. ScholarBank@NUS Repository. | Abstract: | An in vitro culture system for direct shoot regeneration from cotyledon explants of rapid-cycling Brassica rapa was developed. Cotyledons from 3-d-old seedlings, when cultured on Murashige and Skoog (MS) medium supplemented with 20 μM N6-benzyladenine (BA) and 2 μM α-naphthaleneacetic acid (NAA), regenerated shoots directly at a frequency of 20%. The addition of 2 μM aminoethoxyvinylglycine (AVG) to this medium increased shoot regeneration to 33%, but silver nitrate drastically inhibited shoot regeneration. Shoot regeneration occurred directly, at the petiolar cut ends of cotyledonary explants, between 10 to 17 d in culture. The highest percentage of regeneration (33%) was obtained from 3-d-old seedlings. NAA was the most effective auxin for root induction and development, with 49% of shoots producing roots after 2 wk on medium containing 1.0 μM NAA. Regenerated plantlets were grown to maturity in pots containing peat moss and vermiculite (1:1). These plants were morphologically normal and fertile. With this protocol, over 100 independently derived, flowering R0 plants were obtained from 40 regenerating cotyledonary explants within 40 d after culture initiation. | Source Title: | In Vitro Cellular and Developmental Biology - Plant | URI: | http://scholarbank.nus.edu.sg/handle/10635/100474 | ISSN: | 10545476 |
Appears in Collections: | Staff Publications |
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