Please use this identifier to cite or link to this item:
|Title:||Coassembly of REST and its cofactors at sites of gene repression in embryonic stem cells|
|Authors:||Yu, H.-B. |
|Citation:||Yu, H.-B., Johnson, R., Kunarso, G., Stanton, L.W. (2011-08). Coassembly of REST and its cofactors at sites of gene repression in embryonic stem cells. Genome Research 21 (8) : 1284-1293. ScholarBank@NUS Repository. https://doi.org/10.1101/gr.114488.110|
|Abstract:||The differentiation of pluripotent embryonic stem cells is regulated by networks of activating and repressing transcription factors that orchestrate determinate patterns of gene expression. With the recent mapping of target sites for many transcription factors, it has been a conundrum that so few of the genes directly targeted by these factors are transcriptionally responsive to the binding of that factor. To address this, we generated genome-wide maps of the transcriptional repressor REST and five of its corepressors in mouse embryonic stem cells. Combining these binding-site maps with comprehensive gene-expression profiling, we show that REST is functionally heterogeneous. Approximately half of its binding sites apparently are nonfunctional, having weaker binding of REST and low recruitment of corepressors. In contrast, the other sites strongly recruit REST and corepressor complexes with varying numbers of components. Strikingly, the latter sites account for almost all observed gene regulation. These data support a model where productive gene repression by REST requires assembly of a multimeric "repressosome" complex, whereas weak recruitment of REST and its cofactors is insufficient to repress gene expression. © 2011 by Cold Spring Harbor Laboratory Press.|
|Source Title:||Genome Research|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 11, 2018
WEB OF SCIENCETM
checked on Oct 2, 2018
checked on Sep 21, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.