Please use this identifier to cite or link to this item: https://doi.org/10.1021/bc0402888
Title: Chemical conjugation of heterologous proteins on the surface of cowpea mosaic virus
Authors: Chatterji, A.
Ochoa, W.
Shamieh, L.
Salakian, S.P.
Wong, S.M. 
Clinton, G.
Ghosh, P.
Lin, T.
Johnson, J.E.
Issue Date: Jul-2004
Citation: Chatterji, A., Ochoa, W., Shamieh, L., Salakian, S.P., Wong, S.M., Clinton, G., Ghosh, P., Lin, T., Johnson, J.E. (2004-07). Chemical conjugation of heterologous proteins on the surface of cowpea mosaic virus. Bioconjugate Chemistry 15 (4) : 807-813. ScholarBank@NUS Repository. https://doi.org/10.1021/bc0402888
Abstract: Genetic economy leads to symmetric distributions of chemically identical subunits in icosaherdal and helical viruses. Modification of the subunit genes of a variety of viruses has permitted the display of polypeptides on both the infectious virions and virus particles made in expression systems. Icosahedral chimeric particles of this type often display novel properties resulting in high local concentrations of the insert. Here we report an extension of this concept in which entire proteins were chemically cross-linked to lysine and cysteine residues genetically engineered on the coat protein of icosahedral Cowpea mosaic virus particles. Three exogenous proteins, the LRR domain of internalin B, the T4 lysozyme, and the Intron 8 gene product of the of the HER2 tyrosine kinase receptor were derivatized with appropriate bifunctional cross-linkers and conjugated to the virus capsid. Characterization of these particles demonstrated that (1) virtually 100% occupancy of the 60 sites was achieved; (2) biological activity (either enzyme or binding specificity) of the attached protein was preserved; (3) in one case (LRR-internalin B) the attached protein conformed with the icosahedral symmetry to the extent that a reconstruction of the derivatized particles displayed added density with a shape consistent with the X-ray structure of the attached protein. Strategies demonstrated here allow virus particle targeting to specific cell types and the use of an icosahedral virus as a platform for structure determination of small proteins at moderate resolution.
Source Title: Bioconjugate Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/100258
ISSN: 10431802
DOI: 10.1021/bc0402888
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

96
checked on Dec 12, 2018

WEB OF SCIENCETM
Citations

89
checked on Dec 12, 2018

Page view(s)

39
checked on Dec 14, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.