Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.cbpb.2006.08.001
DC Field | Value | |
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dc.title | Characterization of cDNAs encoding cholesterol side chain cleavage and 3β-hydroxysteroid dehydrogenase in the freshwater stingray Potamotrygon motoro | |
dc.contributor.author | Scott Nunez, B. | |
dc.contributor.author | Evans, A.N. | |
dc.contributor.author | Simpson, M.A. | |
dc.contributor.author | Wong, W.P. | |
dc.contributor.author | Ip, Y.K. | |
dc.date.accessioned | 2014-10-27T08:23:34Z | |
dc.date.available | 2014-10-27T08:23:34Z | |
dc.date.issued | 2006-11 | |
dc.identifier.citation | Scott Nunez, B., Evans, A.N., Simpson, M.A., Wong, W.P., Ip, Y.K. (2006-11). Characterization of cDNAs encoding cholesterol side chain cleavage and 3β-hydroxysteroid dehydrogenase in the freshwater stingray Potamotrygon motoro. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 145 (3-4) : 306-317. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cbpb.2006.08.001 | |
dc.identifier.issn | 10964959 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/100238 | |
dc.description.abstract | The interrenal gland (adrenocortical homolog) of elasmobranchs produces a unique steroid, 1α-hydroxycorticosterone (1α-B). The synthesis of this and most other steroids requires both cholesterol side chain cleavage (CYP11A) and 3β-hydroxysteroid dehydrogenase (HSD3). To facilitate the study of elasmobranch steroidogenesis, we isolated complementary DNAs encoding CYP11A and HSD3 from the freshwater stingray Potamotrygon motoro. The P. motoro CYP11A (2182 bp total length) and HSD3 (2248 bp total length) cDNAs harbor open reading frames that encode proteins of 542 and 376 amino acids (respectively) that are similar (CYP11A: 39-61% identical; HSD3: 36-53% identical) to their homologs from other vertebrates. In molecular phylogenetic analysis, P. motoro CYP11A segregates with CYP11A proteins (and not with related CYP11B proteins) and P. motoro HSD3 segregates with steroidogenic HSD3 proteins from other fishes. CYP11A and HSD3 mRNA is found only in interrenal and gonadal tissues, indicating de novo steroidogenesis is restricted to these tissues. Because 1α-B is thought to act in the elasmobranch response to hydromineral disturbances, we examined the effect of adapting P. motoro to 10 ppt seawater on mRNAs encoding steroidogenic genes. The P. motoro response to this salinity challenge does not include interrenal hypertrophy or an increase in the levels of interrenal CYP11A, HSD3 or steroidogenic acute regulatory protein (StAR) mRNA. This study is the first to isolate full length cDNAs encoding elasmobranch CYP11A and HSD3 and the first to examine the regulation of steroidogenic genes in elasmobranch interrenal cells. © 2006 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.cbpb.2006.08.001 | |
dc.source | Scopus | |
dc.subject | 3β-hydroxysteroid dehydrogenase | |
dc.subject | Cytochrome P450 side chain cleavage | |
dc.subject | Elasmobranch | |
dc.subject | Freshwater stingray | |
dc.subject | Interrenal gland | |
dc.subject | Osmoregulation | |
dc.subject | Steroidogenic acute regulatory protein | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1016/j.cbpb.2006.08.001 | |
dc.description.sourcetitle | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology | |
dc.description.volume | 145 | |
dc.description.issue | 3-4 | |
dc.description.page | 306-317 | |
dc.description.coden | CBPBB | |
dc.identifier.isiut | 000243215800007 | |
Appears in Collections: | Staff Publications |
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