Please use this identifier to cite or link to this item:
|Title:||Blag 3: A novel allergen of German cockroach identified using cockroach-specific avian single-chain variable fragment antibody|
|Citation:||Khurana, T., Collison, M., Chew, F.T., Slater, J.E. (2014-02). Blag 3: A novel allergen of German cockroach identified using cockroach-specific avian single-chain variable fragment antibody. Annals of Allergy, Asthma and Immunology 112 (2) : 140-145. ScholarBank@NUS Repository. https://doi.org/10.1016/j.anai.2013.11.007|
|Abstract:||Background The IgE response to cockroach allergens is thought to be associated with asthma. German cockroach (GCr) allergen extract is a complex mixture of allergens, and the identification and characterization of immunodominant allergens is important for the effective diagnosis and treatment of GCr-induced asthma. Objective To characterize a novel GCr allergen homologous to the American cockroach allergen Per a 3. Methods GCr-specific avian monoclonal antibodies were used for direct immunoprecipitation of specific targets from whole-body GCr extract. Precipitated protein was identified by mass spectrometry and sequence analysis. Putative recombinant protein also was expressed, purified, and used for determination of allergenicity, determined by IgE enzyme-linked immunosorbent assay with serum from 61 GCr-allergic patients. The identified target also was analyzed for heat stability using a bead-based assay. Results The immunoprecipitated target of monoclonal antibody 2A1 was identified as a novel allergen of GCr homologous to American cockroach allergen Per a 3. This homolog, designated Bla g 3, has an apparent mass of 78 kDa, can be measured in GCr extract using antibody 2A1, and is a heat-stable protein. Screening of 61 serum samples from GCr-allergic patients showed a 22% prevalence of Bla g 3-specific IgE. Conclusion Bla g 3 is a GCr allergen with structural homology to American cockroach allergen Per a 3. © 2014 American College of Allergy, Asthma and Immunology. Published by Elsevier Inc. All rights reserved.|
|Source Title:||Annals of Allergy, Asthma and Immunology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 23, 2019
WEB OF SCIENCETM
checked on Mar 13, 2019
checked on Mar 1, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.