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|Title:||Blag 3: A novel allergen of German cockroach identified using cockroach-specific avian single-chain variable fragment antibody|
|Citation:||Khurana, T., Collison, M., Chew, F.T., Slater, J.E. (2014-02). Blag 3: A novel allergen of German cockroach identified using cockroach-specific avian single-chain variable fragment antibody. Annals of Allergy, Asthma and Immunology 112 (2) : 140-145. ScholarBank@NUS Repository. https://doi.org/10.1016/j.anai.2013.11.007|
|Abstract:||Background The IgE response to cockroach allergens is thought to be associated with asthma. German cockroach (GCr) allergen extract is a complex mixture of allergens, and the identification and characterization of immunodominant allergens is important for the effective diagnosis and treatment of GCr-induced asthma. Objective To characterize a novel GCr allergen homologous to the American cockroach allergen Per a 3. Methods GCr-specific avian monoclonal antibodies were used for direct immunoprecipitation of specific targets from whole-body GCr extract. Precipitated protein was identified by mass spectrometry and sequence analysis. Putative recombinant protein also was expressed, purified, and used for determination of allergenicity, determined by IgE enzyme-linked immunosorbent assay with serum from 61 GCr-allergic patients. The identified target also was analyzed for heat stability using a bead-based assay. Results The immunoprecipitated target of monoclonal antibody 2A1 was identified as a novel allergen of GCr homologous to American cockroach allergen Per a 3. This homolog, designated Bla g 3, has an apparent mass of 78 kDa, can be measured in GCr extract using antibody 2A1, and is a heat-stable protein. Screening of 61 serum samples from GCr-allergic patients showed a 22% prevalence of Bla g 3-specific IgE. Conclusion Bla g 3 is a GCr allergen with structural homology to American cockroach allergen Per a 3. © 2014 American College of Allergy, Asthma and Immunology. Published by Elsevier Inc. All rights reserved.|
|Source Title:||Annals of Allergy, Asthma and Immunology|
|Appears in Collections:||Staff Publications|
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