Please use this identifier to cite or link to this item: https://doi.org/10.1021/ac2034375
Title: Bayesian approach to the analysis of fluorescence correlation spectroscopy data II: Application to simulated and in vitro data
Authors: Guo, S.-M.
He, J.
Monnier, N.
Sun, G.
Wohland, T. 
Bathe, M.
Issue Date: 1-May-2012
Citation: Guo, S.-M., He, J., Monnier, N., Sun, G., Wohland, T., Bathe, M. (2012-05-01). Bayesian approach to the analysis of fluorescence correlation spectroscopy data II: Application to simulated and in vitro data. Analytical Chemistry 84 (9) : 3880-3888. ScholarBank@NUS Repository. https://doi.org/10.1021/ac2034375
Abstract: Fluorescence correlation spectroscopy (FCS) is a powerful approach to characterizing the binding and transport dynamics of macromolecules. The unbiased interpretation of FCS data relies on the evaluation of multiple competing hypotheses to describe an underlying physical process under study, which is typically unknown a priori. Bayesian inference provides a convenient framework for this evaluation based on the temporal autocorrelation function (TACF), as previously shown theoretically using model TACF curves (He, J., Guo, S., and Bathe, M.Anal. Chem. 2012, 84). Here, we apply this procedure to simulated and experimentally measured photon-count traces analyzed using a multitau correlator, which results in complex noise properties in TACF curves that cannot be modeled easily. As a critical component of our technique, we develop two means of estimating the noise in TACF curves based either on multiple independent TACF curves themselves or a single raw underlying intensity trace, including a general procedure to ensure that independent, uncorrelated samples are used in the latter approach. Using these noise definitions, we demonstrate that the Bayesian approach selects the simplest hypothesis that describes the FCS data based on sampling and signal limitations, naturally avoiding overfitting. Further, we show that model probabilities computed using the Bayesian approach provide a reliability test for the downstream interpretation of model parameter values estimated from FCS data. Our procedure is generally applicable to FCS and image correlation spectroscopy and therefore provides an important advance in the application of these methods to the quantitative biophysical investigation of complex analytical and biological systems. © 2012 American Chemical Society.
Source Title: Analytical Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/100154
ISSN: 00032700
DOI: 10.1021/ac2034375
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

31
checked on Sep 24, 2018

WEB OF SCIENCETM
Citations

31
checked on Sep 17, 2018

Page view(s)

49
checked on Sep 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.