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|Title:||An improved dehydration protocol for cryopreservation of Brassica napus somatic embryos|
|Citation:||Li, C.,Loh, C.-S.,Sun, W.Q. (1999-07). An improved dehydration protocol for cryopreservation of Brassica napus somatic embryos. Cryo-Letters 20 (4) : 263-268. ScholarBank@NUS Repository.|
|Abstract:||This study compared three predehydration methods for cryopreservation of somatic embryos of oilseed rape (Brassica napus L.). These methods were (1) fast-drying in a laminar airflow cabinet at 25°C and 60 to 70% RH, (2) slow-drying for two days in closed jars with constant relative humidities (85%, 75%, 64% and 51%), and (3) stepwise slow-drying over six days using four humidity conditions (97% → 92% → 85% → 75%). Desiccation tolerance of oilseed rape somatic embryos increased significantly after preculture for 7 days in the presence of 10 mg/L abscisic acid (ABA). Under the fast-drying condition, the critical water content of desiccation tolerance (50% survival) for embryos decreased from 1.16 to 0.24 g/g dw after ABA preculture for 7 days. However, fast-dried somatic embryos with water content ranging from 2.51 to 0.16 g/g dw did not survive after freezing and storage in liquid nitrogen. Slow-drying improved the survival after cryopreservation. The highest survival rate (55 ± 3 %) was observed with 75% RH. The best survival rate (81 ± 3 %) was obtained, using the stepwise slow-drying procedure in which RH was progressively lowered from 97 to 75% RH over a period of six days. The data suggest that the drying rate during dehydration greatly affects the survival of somatic embryos after cryopreservation.|
|Appears in Collections:||Staff Publications|
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