Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.febslet.2010.11.060
DC Field | Value | |
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dc.title | An adjacent arginine, and the phosphorylated tyrosine in the c-Met receptor target sequence, dictates the orientation of c-Cbl binding | |
dc.contributor.author | Sun, Q. | |
dc.contributor.author | Ng, C. | |
dc.contributor.author | Guy, G.R. | |
dc.contributor.author | Sivaraman, J. | |
dc.date.accessioned | 2014-10-27T08:21:27Z | |
dc.date.available | 2014-10-27T08:21:27Z | |
dc.date.issued | 2011-01-21 | |
dc.identifier.citation | Sun, Q., Ng, C., Guy, G.R., Sivaraman, J. (2011-01-21). An adjacent arginine, and the phosphorylated tyrosine in the c-Met receptor target sequence, dictates the orientation of c-Cbl binding. FEBS Letters 585 (2) : 281-285. ScholarBank@NUS Repository. https://doi.org/10.1016/j.febslet.2010.11.060 | |
dc.identifier.issn | 00145793 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/100050 | |
dc.description.abstract | Previously, we have demonstrated that the tyrosine phosphorylated hepatocyte growth factor receptor (Met) binds to the c-Cbl phosphotyrosine- recognition, tyrosine kinase binding (TKB) domain in a reverse orientation compared to other c-Cbl binding partners. A Met peptide with the DpYR motif changed to RpYD (MetRD) retains a similar TKB binding affinity as the native Met peptide. However, the TKB: MetRD complex crystal structure reveals a complete reversal of the binding orientation. Collated data indicates that both binding and orientation is dictated by the phosphorylated tyrosine and an adjacent arginine forming intra-peptide hydrogen bonds and aligning unidirectionally with complementary charges in the phosphotyrosine binding pocket of c-Cbl. Structured summary: c-Cbl and MetRD bind: shown by x-ray crystallography (view interaction) c-Cbl and MetRD bind: shown by mass spectrometry studies of complexes (view interaction) c-Cbl bind to Met: shown by surface plasmon resonance (view interactions 1,2). © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.febslet.2010.11.060 | |
dc.source | Scopus | |
dc.subject | c-Casitas B-lineage lymphoma tyrosine kinase binding domain | |
dc.subject | Crystal structure | |
dc.subject | Hepatocyte growth factor receptor | |
dc.subject | Reverse binding | |
dc.subject | Surface plasmon resonance | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1016/j.febslet.2010.11.060 | |
dc.description.sourcetitle | FEBS Letters | |
dc.description.volume | 585 | |
dc.description.issue | 2 | |
dc.description.page | 281-285 | |
dc.description.coden | FEBLA | |
dc.identifier.isiut | 000286180300003 | |
Appears in Collections: | Staff Publications |
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