Please use this identifier to cite or link to this item:
|Title:||Amplified fragment length polymorphism analysis of vandaceous orchids|
Hoon Lim, S.
Man Wong, S.
Hwa Lee, Y.
Wing Yam, T.
|Source:||Chen, X., Hoon Lim, S., Man Wong, S., Hwa Lee, Y., Kuo, J., Wing Yam, T., Lin, J.-J. (1999-02-22). Amplified fragment length polymorphism analysis of vandaceous orchids. Plant Science 141 (2) : 183-189. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-9452(98)00245-3|
|Abstract:||We investigated the application of the PCR-based fingerprinting technique, amplified fragment length polymorphism (AFLP), in orchids. The optimal AFLP patterns have been determined using primer combinations of EcoRI +4 and MseI +3 selective nucleotides. The same reproducible AFLP patterns were demonstrated in genomic DNAs isolated both from: (1) different orchid tissues, e.g. leaves and flowers; and (2) orchid flowers collected at different times. Genomic variations among different cultivars of vandaceous orchid hybrids were successfully determined by AFLP analysis. More than 10% of the AFLP bands were polymorphic DNA when siblings, derived from the same original crosses (two cultivars of Aranda Christine, five cultivars of Mokara Willie How), were used. Only 0.3-0.7% of the AFLP patterns were shown to be polymorphic when different cultivars, originating from somatic mutations during meristem culture for massive propagation, were used (two cultivars of Ar. Christine, four cultivars of M. Chark Kuan).|
|Source Title:||Plant Science|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 21, 2018
WEB OF SCIENCETM
checked on Jan 15, 2018
checked on Feb 18, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.