Please use this identifier to cite or link to this item: https://doi.org/10.1089/hum.2005.16.1219
Title: Adeno-associated virus inverted terminal repeats improve neuronal transgene expression mediated by baculoviral vectors in rat brain
Authors: Wang, C.-Y.
Wang, S. 
Issue Date: Oct-2005
Citation: Wang, C.-Y., Wang, S. (2005-10). Adeno-associated virus inverted terminal repeats improve neuronal transgene expression mediated by baculoviral vectors in rat brain. Human Gene Therapy 16 (10) : 1219-1226. ScholarBank@NUS Repository. https://doi.org/10.1089/hum.2005.16.1219
Abstract: Baculoviral vectors can transduce neurons in the CNS but mediate only transient expression of transgenes. We have developed a new baculoviral vector in which the inverted terminal repeats (ITRs) of adeno-associated virus are used to flank a luciferase reporter gene cassette harboring a neuron-specific promoter. When tested in rat brain, the new viral vector was able to provide transgene expression for at least 90 days. Immunohistological analysis demonstrated that ITR flanking did not affect the cellular preference of the neuronal promoter in the context of baculovirus. These findings establish an effective way to engineer baculoviral vectors in order to achieve sustained expression of a functional gene for gene therapy for neurodegenerative disorders and physiological studies of neurons. © Mary Ann Liebert, Inc.
Source Title: Human Gene Therapy
URI: http://scholarbank.nus.edu.sg/handle/10635/100013
ISSN: 10430342
DOI: 10.1089/hum.2005.16.1219
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